The PHKA1 Polyclonal Antibody (CAB15700) is a valuable tool for researchers studying PHKA1, a key enzyme involved in glycogen metabolism. This antibody, generated in rabbits, is highly specific and reacts with human samples, allowing for accurate detection and analysis in various experimental settings. Validated for use in Western blot applications, the PHKA1 Polyclonal Antibody binds specifically to the PHKA1 protein, enabling researchers to study its role in metabolic pathways and diseases related to glycogen storage disorders.
PHKA1 is essential for the regulation of glycogen synthesis and breakdown in the body, making it a crucial target for understanding metabolic disorders such as Glycogen Storage Disease type IX. By studying the function and activity of PHKA1, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially develop novel therapeutic strategies for their management. The PHKA1 Polyclonal Antibody is a reliable tool for exploring the biology of PHKA1 and its implications for human health.
Product Name:
PHKA1 Rabbit Polyclonal Antibody
SKU:
CAB15700
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 600-820 of human PHKA1 (NP_001116142.1).
Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9D, also known as X-linked muscle glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. A pseudogene has been found on chromosome 1.
Purification Method:
Affinity purification
Gene ID:
5255
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PHKA1 Rabbit pAb (CAB15700) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
