The PHGDH Polyclonal Antibody (CAB10461) is a valuable tool for researchers studying the enzyme PHGDH, which plays a key role in the serine biosynthesis pathway. This antibody, produced in rabbits, is highly specific and reactive to human samples, making it ideal for applications such as Western blotting.PHGDH is an essential enzyme involved in cancer metabolism, as it is often upregulated in cancer cells to support their rapid growth. By targeting PHGDH with this antibody, researchers can gain insights into the role of this enzyme in cancer progression and potentially identify new therapeutic targets.
In addition to its relevance in cancer research, PHGDH has also been implicated in other disease contexts, such as neurodegenerative disorders and metabolic diseases. The PHGDH Polyclonal Antibody (CAB10461) offers researchers a reliable tool to study the expression and activity of PHGDH in various biological samples, ultimately advancing our understanding of the role of this enzyme in health and disease.
Product Name:
PHGDH Rabbit Polyclonal Antibody
SKU:
CAB10461
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 264-533 of human PHGDH (NP_006614.2).
This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.
Purification Method:
Affinity purification
Gene ID:
26227
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PHGDH Rabbit pAb (CAB10461) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
