The PHF1 Polyclonal Antibody (CAB8450) is a valuable tool for researchers studying PHF1, a key protein involved in chromatin organization and gene regulation. This antibody, produced in rabbits, is highly specific for PHF1 and has been validated for use in various applications, including Western blotting. By binding to PHF1, this antibody allows for the detection and analysis of PHF1 protein levels in different cell types, making it ideal for studies in epigenetics and cancer research.PHF1 is a member of the Polycomb group proteins, which play a crucial role in maintaining gene silencing and regulating cell differentiation.
Dysregulation of PHF1 has been implicated in several diseases, including cancer and developmental disorders. By studying PHF1, researchers can gain insight into the mechanisms underlying these conditions and potentially identify new therapeutic targets.Overall, the PHF1 Polyclonal Antibody (CAB8450) provides researchers with a reliable tool to investigate the role of PHF1 in chromatin modification and gene expression, paving the way for advancements in understanding epigenetic regulation and developing targeted therapies for disease.
Product Name:
PHF1 Rabbit Polyclonal Antibody
SKU:
CAB8450
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PHF1 (NP_077084.1).
This gene encodes a Polycomb group protein. The protein is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex, and functions in transcriptional repression of homeotic genes. The protein is also recruited to double-strand breaks, and reduced protein levels results in X-ray sensitivity and increased homologous recombination. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5252
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PHF1 antibody (CAB8450) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
