The PHD2/EGLN1 Monoclonal Antibody is a valuable tool for researchers studying the PHD2/EGLN1 protein, which plays a key role in oxygen sensing and regulation of cellular responses to hypoxia. This monoclonal antibody, generated through hybridoma technology, offers high specificity and sensitivity for detecting PHD2/EGLN1 in human samples, making it ideal for use in immunohistochemistry, immunofluorescence, and other experimental techniques.PHD2/EGLN1 is a member of the prolyl hydroxylase domain protein family and is involved in the hydroxylation of hypoxia-inducible factor (HIF), a transcription factor that regulates the expression of genes involved in oxygen homeostasis.
Dysregulation of PHD2/EGLN1 has been linked to various diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions, making it a promising target for therapeutic interventions.By utilizing the PHD2/EGLN1 Monoclonal Antibody in their research, scientists can gain insights into the molecular mechanisms underlying hypoxia response and potentially identify new strategies for treating hypoxia-related diseases. This antibody is a valuable tool for advancing our understanding of PHD2/EGLN1 function and its implications for human health.
Product Name:
PHD2/EGLN1 Monoclonal Antibody
SKU:
CAB21252
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PHD2/EGLN1 (NP_071334.1).
Sequence:
MAND SGGP GGPS PSER DRQY CELC GKME NLLR CSRC RSSF YCCK EHQR QDWK KHKL VCQG SEGA LGHG VGPH QHSG PAPP AAVP PPRA GARE PRKA AARR
The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).
Purification Method:
Affinity purification
Gene ID:
54583
Clone Number:
ARC52542
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts from normal (control) and PHD2/EGLN1 knockout (KO) 293T(KO) cells, using PHD2/EGLN1 antibody (CAB21252) at 1:20000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 8s.
