The PHD1 Prolyl Hydroxylase Rabbit Monoclonal Antibody (CAB3730) is a highly specific antibody designed for research involving PHD1, a prolyl hydroxylase enzyme that plays a critical role in the regulation of cellular responses to hypoxia. This antibody, raised in rabbits, is optimized for detection of PHD1 protein in human samples and has been validated for use in Western blot applications.PHD1 is a key enzyme involved in the cellular response to low oxygen levels, known as hypoxia. It regulates the stability of hypoxia-inducible factors (HIFs), which control the expression of genes involved in processes such as angiogenesis, erythropoiesis, and metabolism.
Dysfunction of PHD1 has been implicated in a variety of diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions.The PHD1 Prolyl Hydroxylase Rabbit Monoclonal Antibody (CAB3730) enables accurate detection and analysis of PHD1 protein levels in various cell types, making it an invaluable tool for researchers studying the role of PHD1 in hypoxia signaling pathways and its implications for disease pathology. By understanding the function of PHD1, researchers can gain insights into potential therapeutic targets for diseases characterized by dysregulated oxygen sensing mechanisms.
The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.
Purification Method:
Affinity purification
Gene ID:
112398
Clone Number:
ARC0230
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Prolyl hydroxylase PHD1 (EGLN2) Rabbit mAb (CAB3730) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
