The Phax Polyclonal Antibody (PACO4576) is a valuable tool for researchers studying the PHAX protein, which plays a crucial role in the processing and maturation of small nucleolar RNAs (snoRNAs). This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the PHAX protein, researchers can effectively detect and analyze PHAX expression in various cell types, making it an essential component for studies in molecular biology and RNA processing.PHAX is essential for the transportation of snoRNAs from the nucleolus to the cytoplasm, where they participate in ribosome biogenesis and other critical cellular processes.
Dysregulation of PHAX function has been linked to various diseases, including cancer and neurodevelopmental disorders. As such, studying the role of PHAX in RNA processing and cellular function is key to advancing our understanding of these conditions and developing potential therapeutic interventions.The Phax Polyclonal Antibody offers researchers a reliable tool to explore the intricate mechanisms underlying snoRNA processing and the potential implications for disease pathology. Its high specificity and sensitivity make it an ideal choice for investigating PHAX expression and function in a wide range of experimental settings, providing valuable insights into the intricate world of RNA biology.
Antibody Name:
PHAX Antibody
Antibody SKU:
PACO04576
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
WB:1:500-1:2000, IHC:1:200-1:1000
Species Reactivity:
Human
Immunogen:
synthesized peptide derived from the Internal region of human PHAX.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
PHAX; RNUXA; Phosphorylated adapter RNA export protein; RNA U small nuclear RNA export adapter protein
UniProt Protein Function:
RNUXA: A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation- independent manner. Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA. Belongs to the PHAX family.Protein type: RNA-binding; Nuclear importChromosomal Location of Human Ortholog: 5q23.2Cellular Component: centrosome; cytosol; intracellular membrane-bound organelle; nucleoplasm; nucleusMolecular Function: protein bindingBiological Process: nuclear export; snRNA export from nucleus; snRNA transcription from RNA polymerase II promoter
