The PGM1 Polyclonal Antibody (CAB6303) is a powerful tool for researchers studying PGM1, an enzyme involved in carbohydrate metabolism. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the PGM1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for investigations in biochemistry and metabolic research.PGM1, or phosphoglucomutase 1, is essential for the conversion of glucose-1-phosphate to glucose-6-phosphate, a key step in carbohydrate utilization.
Dysregulation of PGM1 activity has been linked to metabolic disorders and diseases such as glycogen storage disease. Understanding the function of PGM1 can provide valuable insights into these conditions and may lead to the development of targeted therapies.By using the PGM1 Polyclonal Antibody in your research, you can unlock the potential of this enzyme and further our understanding of carbohydrate metabolism. This antibody is a valuable resource for scientists investigating metabolic pathways and seeking to unravel the complexities of metabolic diseases.
Antibody Name:
Anti-PGM1 Antibody
Antibody SKU:
CAB6303
Antibody Size:
20uL, 50uL, 100uL
Application:
WB IF
Reactivity:
Human, Mouse, Rat
Host Species:
Rabbit
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PGM1 (NP_002624.2).
Application:
WB IF
Recommended Dilution:
WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:
Human, Mouse, Rat
Positive Samples:
U-251MG, HepG2, 293T, Mouse liver, Mouse heart, Rat liver
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PGM1 (NP_002624.2).
Purification Method:
Affinity purification
Storage Buffer:
Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.
UniProt Protein Function:
This enzyme participates in both the breakdown and synthesis of glucose.
NCBI Summary:
The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Mar 2010]
Western blot analysis of extracts of various cell lines, using PGM1 antibody (CAB6303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Immunofluorescence analysis of U2OS cells using PGM1 antibody (CAB6303). Blue: DAPI for nuclear staining.
