The PGK1 Rabbit Monoclonal Antibody offered by Assay Genie is a high-quality research tool designed for studying the PGK1 protein. PGK1 is a key enzyme involved in glycolysis and is upregulated in various cancers, making it an important target for cancer research.This antibody, generated in rabbits, is highly specific and sensitive for detecting PGK1 in human samples. It has been validated for use in Western blot applications, enabling researchers to analyze the expression levels of PGK1 in different cell types and tissues.
PGK1 plays a crucial role in tumor metabolism and growth, making it a potential target for cancer therapy. By understanding the expression and function of PGK1, researchers can gain insights into metabolic reprogramming in cancer cells and develop novel treatment strategies.Overall, the PGK1 Rabbit Monoclonal Antibody is a valuable tool for researchers studying cancer metabolism and seeking to uncover new therapeutic targets for cancer treatment.
Product Name:
PGK1 Rabbit Monoclonal Antibody
SKU:
CAB12686
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PGK1 (P00558).
The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome.
Purification Method:
Affinity purification
Gene ID:
5230
Clone Number:
ARC0700
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PGK1 Rabbit mAb (CAB12686) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
