The PGE2 (Prostaglandin E2) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of PGE2 levels in various biological samples such as serum, plasma, and cell culture supernatants. This kit ensures reliable and reproducible results, making it suitable for a wide range of research applications.Prostaglandin E2 is a key mediator of inflammation, pain, and fever, playing a crucial role in a variety of physiological and pathological processes. By measuring PGE2 levels, researchers can gain valuable insights into the mechanisms underlying inflammatory diseases, cancer, and other health conditions, paving the way for the development of novel therapeutic strategies.
With its user-friendly protocol and excellent performance characteristics, the PGE2 ELISA Kit from Assay Genie is an indispensable tool for studying the role of prostaglandins in health and disease. Get accurate and dependable results with this cutting-edge assay kit.
Product Name:
PGE2 (Prostaglandin E2) ELISA Kit (UNES00024)
SKU:
UNES00024
Target:
PGE2 (Prostaglandin E2) (UNES00024)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PGE2. During the reaction, PGE2 in the sample or standard competes with a fixed amount of PGE2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to PGE2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PGE2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
91-104
89-101
Average (%)
103
98
96
1:4
Range (%)
85-95
89-101
101-117
Average (%)
90
95
107
1:8
Range (%)
87-100
92-104
96-111
Average (%)
92
97
102
1:16
Range (%)
88-104
93-105
99-114
Average (%)
95
98
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-109
99
EDTA plasma (n=5)
87-99
94
Cell culture media (n=5)
86-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.3
236.8
779.3
96.0
259.9
728.6
Standard deviation
6.6
11.6
42.1
4.9
13.8
31.3
C V (%)
6.52
4.9
5.4
5.1
5.31
4.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of PGE2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes PGE2 in samples. No significant cross-reactivity or interference between PGE2 and analogues was observed.
