The PGAM1 Polyclonal Antibody (CAB4015) is a valuable tool for researchers interested in studying the PGAM1 protein, a key enzyme involved in glycolysis and energy metabolism in cells. This antibody, generated in rabbits, is highly specific to human PGAM1 and has been validated for use in Western blot applications. By binding to PGAM1, the antibody allows for the detection and analysis of this important enzyme in various cell types, making it ideal for studies in metabolism, cancer biology, and other related fields.PGAM1, also known as phosphoglycerate mutase 1, plays a crucial role in the glycolytic pathway by catalyzing the conversion of 3-phosphoglycerate to 2-phosphoglycerate.
Dysregulation of PGAM1 has been implicated in various diseases, including cancer, metabolic disorders, and neurological conditions. Understanding the function and regulation of PGAM1 is therefore essential for developing targeted therapies and diagnostic tools for these conditions.Overall, the PGAM1 Polyclonal Antibody offers researchers a reliable and specific tool for investigating the role of PGAM1 in cellular metabolism and disease pathogenesis. Its high reactivity and specificity make it a valuable asset for studies aiming to unravel the complex and critical functions of this enzyme.
Product Name:
PGAM1 Rabbit Polyclonal Antibody
SKU:
CAB4015
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-254 of human PGAM1 (NP_002620.1).
cytoplasm, cytosol, extracellular exosome, extracellular region
Calculated MW:
29kDa
Observed MW:
29kDa
The protein encoded by this gene is a mutase that catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5223
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PGAM1 antibody (CAB4015) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
