Anti-PGA5 Antibody (CAB12951)

Antibody Name:	Anti-PGA5 Antibody
Antibody SKU:	CAB12951
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 63-180 of human PGA5 (NP_055039.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HT-29, 293T, Mouse brain, Mouse stomach, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 63-180 of human PGA5 (NP_055039.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VDEQ PLEN YLDM EYFG TIGI GTPA QDFT VVFD TGSS NLWV PSVY CSSL ACTN HNRF NPED SSTY QSTS ETVS ITYG TGSM TGIL GYDT VQVG GISD TNQI FGLS ETEP GSFL YYAP FD
Gene ID:	5222
Uniprot:	P0DJD9
Cellular Location:	Secreted
Calculated MW:	41kDa
Observed MW:	42kDa

Synonyms:

PGA5, Pg5

Background:

This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer.

UniProt Protein Function:	PGA5: Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. Belongs to the peptidase A1 family.
UniProt Protein Details:	Protein type:EC 3.4.23.1; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 11q13 Molecular Function:aspartic-type endopeptidase activity; peptidase activity Biological Process: cellular protein metabolic process; protein catabolic process; proteolysis
NCBI Summary:	This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer. [provided by RefSeq, Jul 2015]
UniProt Code:	P0DJD9
NCBI GenInfo Identifier:	378522017
NCBI Gene ID:	5222
NCBI Accession:	P0DJD9.1
UniProt Secondary Accession:	P0DJD9,P00790, Q7M4R0, Q8N1E3, A8K749, B7ZW62, B7ZW75
UniProt Related Accession:	P0DJD9
Molecular Weight:	41,993 Da
NCBI Full Name:	Pepsin A-5
NCBI Synonym Full Names:	pepsinogen 5, group I (pepsinogen A)
NCBI Official Symbol:	PGA5
NCBI Official Synonym Symbols:	Pg5
NCBI Protein Information:	pepsin A-5
UniProt Protein Name:	Pepsin A-5
UniProt Synonym Protein Names:	Pepsinogen-5
Protein Family:	Pepsin
UniProt Gene Name:	PGA5
UniProt Entry Name:	PEPA5_HUMAN

Western blot analysis of extracts of various cell lines, using PGA5 antibody (CAB12951) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)