The PFO Polyclonal Antibody (PAC050258) is a crucial tool for researchers studying the role of PFO (perfringolysin O) in immune response and cell permeabilization. This antibody, developed through the use of rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the PFO protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential asset for research in immunology and infectious diseases.PFO is a key component of the toxin produced by certain clostridial species, including Clostridium perfringens.
This protein plays a critical role in membrane permeabilization and cytolysis, leading to cell death. Understanding the mechanisms by which PFO functions is vital in the development of treatments for infections caused by these bacteria. Additionally, research into PFO's role in immune response modulation could provide valuable insights for the treatment of diseases such as sepsis and other inflammatory conditions.Order the PFO Polyclonal Antibody (PAC050258) today and advance your research in immunology and infectious diseases.
Antibody Name:
pfo Antibody (PACO50258)
Antibody SKU:
PACO50258
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:5000
Species Reactivity:
Clostridium perfringens
Immunogen:
Recombinant Clostridium perfringens Perfringolysin O protein (29-500AA)
Western Blot. Positive WB detected in Recombinant protein. All lanes: pfo antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 56 kDa. Observed band size: 56 kDa..
Background:
Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A conformation change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol may be required for binding to host cell membranes, membrane insertion and pore formation. Can be reversibly inactivated by oxidation.
Synonyms:
Perfringolysin O (Theta-toxin) (Thiol-activated cytolysin), pfo, pfoA pfoR
UniProt Protein Function:
Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A conformation change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol may be required for binding to host cell membranes, membrane insertion and pore formation. Can be reversibly inactivated by oxidation.
