The PFKM Polyclonal Antibody (CAB5477) is a valuable tool for researchers studying PFKM, a key enzyme involved in glycolysis and energy production in cells. This antibody, raised in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By binding to the PFKM protein, this antibody allows for the detection and analysis of PFKM expression in various cell types, making it ideal for studies in metabolism, cancer, and cellular energy regulation.PFKM, also known as phosphofructokinase, is essential for the conversion of glucose to energy in cells, making it a crucial player in metabolism and cell growth.
Dysregulation of PFKM has been implicated in various diseases, including cancer and metabolic disorders, highlighting the importance of studying this enzyme in cellular function and disease pathology. The PFKM Polyclonal Antibody provides researchers with a reliable tool to further explore the role of PFKM in these contexts and potentially uncover new therapeutic targets.
Product Name:
PFKM Rabbit Polyclonal Antibody
SKU:
CAB5477
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 501-780 of human PFKM (NP_001160160.1).
Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.
Purification Method:
Affinity purification
Gene ID:
5213
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PFKM antibody (CAB5477) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
