The PFKFB2 Polyclonal Antibody (CAB9311) is a valuable tool for researchers studying PFKFB2, a key enzyme involved in glucose metabolism and cancer progression. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the PFKFB2 protein, researchers can accurately detect and analyze its expression in a variety of cell types, making it ideal for studies in cancer research and metabolic disorders.PFKFB2, a critical regulator of glycolysis, is known to play a pivotal role in tumor growth and survival by promoting the Warburg effect.
Its involvement in metabolic reprogramming makes it a promising target for therapeutic intervention in cancer treatment. Furthermore, understanding the molecular mechanisms underlying PFKFB2 activity can provide insights into metabolic dysregulation in diseases such as diabetes and cardiovascular disorders. Researchers can leverage the power of the PFKFB2 Polyclonal Antibody to further explore the functions of PFKFB2 and its implications in disease pathology.
Product Name:
PFKFB2 Rabbit Polyclonal Antibody
SKU:
CAB9311
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 446-505 of human PFKFB2 (NP_006203.2).
The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5208
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PFKFB2 antibody (CAB9311) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
