The PFKFB1/ PFKFB4 Antibody (PAC021867) is a valuable tool for researchers studying glycolysis and cancer metabolism. This polyclonal antibody is designed to specifically target and detect the PFKFB1 and PFKFB4 proteins, key regulators of glucose metabolism in cells. Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications.PFKFB1 and PFKFB4 are enzymes that play a crucial role in the regulation of glycolysis, the process by which cells convert glucose into energy. Dysregulation of glycolysis is a common feature of many types of cancer, making these enzymes potential targets for anti-cancer therapies.
By using the PFKFB1/ PFKFB4 Antibody, researchers can study the expression and activity of these enzymes in various cell types, providing insights into the role of glycolysis in cancer development and progression.This antibody is an essential tool for studies in cancer research, metabolism, and drug development. By understanding the function of PFKFB1 and PFKFB4 in cellular metabolism, researchers can uncover new potential targets for therapeutic intervention in cancer and other metabolic disorders.
Antibody Name:
PFKFB1/PFKFB4 Antibody (PACO21867)
Antibody SKU:
PACO21867
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthesized peptide derived from internal of human PFKFB1/4.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from HeLa cells, HepG2 cells, COLO205 cells and 293 cells, using PFKFB1/4 antibody.
Background:
Synthesis and degradation of fructose 2,6-bisphosphate. Sakai A., J. Biochem. 119:506-511(1996).
This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]
