Anti-PFDN5 Antibody (CAB4014)

Antibody Name:	Anti-PFDN5 Antibody
Antibody SKU:	CAB4014
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-154 of human PFDN5 (NP_002615.2).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Raji, U-87MG, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-154 of human PFDN5 (NP_002615.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAQS INIT ELNL PQLE MLKN QLDQ EVEF LSTS IAQL KVVQ TKYV EAKD CLNV LNKS NEGK ELLV PLTS SMYV PGKL HDVE HVLI DVGT GYYV EKTA EDAK DFFK RKID FLTK QMEK IQPA LQEK HAMK QAVM EMMS QKIQ QLTA LGAA QATA KA
Gene ID:	5204
Uniprot:	Q99471
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	7kDa/12kDa/17kDa
Observed MW:	17kDa

Synonyms:

PFDN5, MM-1, MM1, PFD5

Background:

This gene encodes a member of the prefoldin alpha subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. The encoded protein may also repress the transcriptional activity of the proto-oncogene c-Myc. Alternatively spliced transcript variants encoding different isoforms have been described.

UniProt Protein Function:	PFDN5: Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Represses the transcriptional activity of MYC. Belongs to the prefoldin subunit alpha family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 12q12 Cellular Component: cytoplasm; nucleus; prefoldin complex Molecular Function:protein binding; unfolded protein binding; transcription corepressor activity Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; regulation of transcription, DNA-dependent; protein folding; retina development in camera-type eye; negative regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a member of the prefoldin alpha subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. The encoded protein may also repress the transcriptional activity of the proto-oncogene c-Myc. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99471
NCBI GenInfo Identifier:	12231038
NCBI Gene ID:	5204
NCBI Accession:	Q99471.2
UniProt Related Accession:	Q99471
Molecular Weight:	17kDa
NCBI Full Name:	Prefoldin subunit 5
NCBI Synonym Full Names:	prefoldin subunit 5
NCBI Official Symbol:	PFDN5
NCBI Official Synonym Symbols:	MM1; MM-1; PFD5
NCBI Protein Information:	prefoldin subunit 5
UniProt Protein Name:	Prefoldin subunit 5
UniProt Synonym Protein Names:	C-Myc-binding protein Mm-1; Myc modulator 1
Protein Family:	Prefoldin
UniProt Gene Name:	PFDN5
UniProt Entry Name:	PFD5_HUMAN

	Western blot analysis of extracts of various cell lines, using PFDN5 antibody (CAB4014) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunofluorescence analysis of C6 cells using PFDN5 Rabbit pAb (CAB4014) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using PFDN5 Rabbit pAb (CAB4014) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using PFDN5 Rabbit pAb (CAB4014) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

PFDN5 Antibody (PACO11182)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)