The PFDN4 Polyclonal Antibody (CAB15300) is a powerful tool for researchers studying the PFDN4 protein, a component of the molecular chaperone complex involved in protein folding and assembly. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting PFDN4 in human samples, making it an essential tool for Western blot applications.PFDN4, also known as prefoldin subunit 4, plays a crucial role in maintaining protein homeostasis and preventing protein misfolding, which can lead to cellular dysfunction and disease. Its involvement in protein folding processes makes it a key player in various cellular functions, including cell growth, differentiation, and apoptosis.
By using the PFDN4 Polyclonal Antibody, researchers can gain valuable insights into the molecular mechanisms underlying protein folding and assembly, as well as the role of PFDN4 in cellular processes. This antibody is an invaluable resource for studies in cell biology, biochemistry, and molecular biology, providing a deeper understanding of protein quality control mechanisms and potential therapeutic targets for protein folding-related diseases.
Product Name:
PFDN4 Rabbit Polyclonal Antibody
SKU:
CAB15300
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-134 of human PFDN4 (NP_002614.2).
Sequence:
MAAT MKKA AAED VNVT FEDQ QKIN KFAR NTSR ITEL KEEI EVKK KQLQ NLED ACDD IMLA DDDC LMIP YQIG DVFI SHSQ EETQ EMLE EAKK NLQE EIDA LESR VESI QRVL ADLK VQLY AKFG SNIN LEAD ES
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:200 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:
C1; PFD4; PFDN4
Positive Sample:
DU145,HeLa,293T,MCF7,mouse kidney
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Mitochondrion, Nucleus.
Calculated MW:
15kDa
Observed MW:
16kDa
This gene encodes a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils.
Purification Method:
Affinity purification
Gene ID:
5203
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PFDN4 Rabbit pAb (CAB15300) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
