The Perforin-1 Polyclonal Antibody (CAB0093) is a valuable tool for researchers studying the perforin-1 protein, a key component of the immune system involved in cell-mediated cytotoxicity. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the perforin-1 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for investigations in immunology and cancer research.Perforin-1 plays a crucial role in activating cell death in target cells, particularly those infected by viruses or cancerous cells.
Understanding the function of perforin-1 is essential for unraveling the mechanisms of immune surveillance and defense against pathogens and abnormal cells. Research on perforin-1 could lead to insights into immune-related diseases such as cancer, viral infections, and autoimmune disorders, paving the way for the development of targeted therapies to enhance immune responses and combat these conditions effectively.
Product Name:
Perforin Rabbit Polyclonal Antibody
SKU:
CAB0093
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 280-555 of human Perforin (NP_005032.2).
This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood.
Purification Method:
Affinity purification
Gene ID:
5551
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat thymus using Perforin Rabbit pAb (CAB0093) at 1:1000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
