PerCP/Cyanine5.5 Mouse IgG3, κ Isotype Control [A112-3]
The PerCP-Cyanine5.5 Mouse IgG3 Isotype Control (AGEL2875) is a crucial tool for immunological research, specifically designed as a negative control for flow cytometry experiments. This isotype control antibody, validated for use in flow cytometry applications, is essential for determining non-specific binding and background signal in samples stained with PerCP-Cyanine5.5-conjugated antibodies.In flow cytometry, it is crucial to distinguish specific antibody binding from non-specific interactions, and using an isotype control like AGEL2875 helps researchers to accurately interpret their experimental results. This Mouse IgG3 Isotype Control binds non-specifically to cells, mirroring the background staining encountered with specific antibodies, providing a baseline for setting gates and thresholds during flow cytometry analysis.
AGEL2875 ensures reliable and reproducible flow cytometry data by serving as a reference for comparison with samples stained with PerCP-Cyanine5.5-conjugated primary antibodies targeting specific antigens. With its high specificity and consistent performance, this isotype control contributes to the accuracy and reliability of flow cytometry experiments in immunology, cell biology, and cancer research.
Product Name:
PerCP/Cyanine5.5 Mouse IgG3, κ Isotype Control [A112-3] (AGEL2875)
Product Code:
AGEL2875
Clone No:
A112-3
Host Species:
Mouse
Applications:
FCM
Isotype:
Mouse IgG3, κ
Conjugation:
PerCP/Cyanine5.5
Form:
Liquid
Fluorescent Label:
PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.