The PERCP-Cyanine5.5 Anti-Mouse CD4 Antibody (RM4-5) is a crucial tool for researchers studying CD4, a key cell surface marker involved in immune response regulation. This monoclonal antibody, optimized for use in flow cytometry applications, specifically targets the CD4 protein found on mouse cells, enabling precise identification and analysis of CD4-positive cell populations.CD4 is a well-known marker for T helper cells, playing a central role in orchestrating immune responses and contributing to various cellular functions. The PERCP-Cyanine5.5 Anti-Mouse CD4 Antibody (RM4-5) offers high specificity and sensitivity in detecting CD4-expressing cells, making it an essential reagent for immunology studies, infectious disease research, and investigations into autoimmune disorders.
By utilizing this antibody, researchers can gain valuable insights into the distribution, activation, and function of CD4-positive T cells in various experimental models. The ability to accurately label CD4 cells with PERCP-Cyanine5.5 facilitates detailed flow cytometric analysis, paving the way for advancements in understanding immune regulation, disease mechanisms, and therapeutic interventions targeting CD4-mediated pathways.
PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Control[2A3]
Form:
Liquid
Conjugation:
PerCP/Cyanine 5.5
Recommended Use:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Swissprot:
P06332
Gene ID:
12504
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.