The PerCP-Cyanine5.5 Anti-Human CD25 Antibody (BC96) from AssayGenie is a high-quality antibody designed for research applications involving CD25, a cell surface marker commonly associated with regulatory T cells. This antibody is specifically targeted for human samples and is validated for use in flow cytometry and immunofluorescence assays.CD25, also known as the interleukin-2 receptor alpha chain, plays a crucial role in immune regulation by promoting the expansion and function of regulatory T cells, which are key players in maintaining immune tolerance and preventing autoimmunity.
Understanding the expression and function of CD25 is essential for studying immune responses in various contexts, including autoimmune diseases, transplantation, and cancer.The PerCP-Cyanine5.5 Anti-Human CD25 Antibody (BC96) offers excellent sensitivity and specificity in detecting CD25-expressing cells, making it a valuable tool for researchers in immunology, oncology, and related fields. With its ability to accurately identify and quantify CD25-positive cell populations, this antibody facilitates in-depth analysis of immune responses and the development of targeted therapies.
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
P01589
Gene ID:
3559
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.