The Mouse CD90.2 Monoclonal Antibody (30-H12) offered by Assay Genie is a vital tool for researchers studying mouse CD90.2, a cell surface marker commonly used to identify hematopoietic stem cells and other cell types. This monoclonal antibody, produced through hybridoma technology, exhibits high specificity and sensitivity for mouse samples, making it an excellent choice for flow cytometry, immunofluorescence, and immunohistochemistry applications.CD90.2, also known as Thy1.2, is a well-characterized marker for various cell populations, including T cells, thymocytes, and stem cells. Its presence on the cell surface is indicative of certain cellular functions and differentiation stages, making it an essential molecule in immunology and stem cell research.
By utilizing the 30-H12 antibody, researchers can accurately detect and quantify CD90.2 expression in different tissues and cell populations, aiding in the characterization and isolation of specific cell subsets.Furthermore, the versatility of the Mouse CD90.2 Monoclonal Antibody (30-H12) allows for its application in diverse experimental settings, providing valuable insights into cell biology, immunophenotyping, and stem cell research. With its reliable performance and proven compatibility with various techniques, this antibody serves as a valuable resource for investigating the roles and functions of CD90.2 in mouse models, contributing to a better understanding of cell differentiation processes, immune responses, and disease mechanisms.
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Gene ID:
21838
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30H12 antibody has been reported to induce Ca2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.