Pepsinogen II (PG II) Monoclonal Antibody (CAB22281)
Pepsinogen II (PG II) Monoclonal Antibody (CAB22281) is a specialized antibody designed for research involving the detection and analysis of pepsinogen II, a precursor to the digestive enzyme pepsin. This monoclonal antibody, developed through advanced techniques, is highly specific and reactive with human samples, making it ideal for studies in gastroenterology and related fields.Pepsinogen II is primarily produced in the stomach and plays a crucial role in the digestion process by aiding in the breakdown of proteins. Abnormal levels of pepsinogen II have been associated with various gastric conditions, including gastritis and gastric cancer, making it a valuable biomarker for diagnostic and research purposes.
Using the PG II Monoclonal Antibody, researchers can accurately detect and quantify pepsinogen II in biological samples, enabling the study of its expression patterns and potential implications in gastrointestinal health. This antibody is validated for use in techniques such as Western blotting, immunofluorescence, and immunohistochemistry, providing versatile applications for investigating pepsinogen II in different experimental settings.
Product Name:
Pepsinogen II (PG II) Monoclonal Antibody
SKU:
CAB22281
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-388 of human Pepsinogen II (PG II) (NP_002621.1).
This gene encodes an aspartic proteinase that belongs to the peptidase family A1. The encoded protein is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is also secreted into the serum. This protein is synthesized as an inactive zymogen that includes a highly basic prosegment. This enzyme is converted into its active mature form at low pH by sequential cleavage of the prosegment that is carried out by the enzyme itself. Polymorphisms in this gene are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1.
Purification Method:
Affinity purification
Gene ID:
5225
Clone Number:
ARC57719
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Pepsinogen II (PG II) Rabbit mAb (CAB22281) at1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
