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PEPCK/PCK2 Polyclonal Antibody (CAB21529)

SKU:
CAB21529
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
IF
IHC
IP
€139 - €529
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:PEPCK/PCK2 Polyclonal Antibody
SKU:CAB21529
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PEPCK/PCK2 (NP_004554.2).
Sequence:MAAL YRPG LRLN WHGL SPLG WPSC RSIQ TLRV LSGD LGQL PTGI RDFV EHSA RLCQ PEGI HICD GTEA ENTA TLTL LEQQ GLIR KLPK YNNC WLAR TDPK DVAR VESK TVIV TPSQ RDTV QLPP GGAR GQLG NWMS PADF QRAV DERF PGCM QGRT MYVL PFSM GPVG SPLS RIGV QLTD SAYV VASM RIMT RLGT PVLQ ALGD GDFV KCLH SVGQ PLTG QGEP VSQW PCNP EKTL IGHV PDQR EIIS FGSG YGGN SLLG KKCF ALRI ASRL ARDE GWLA
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:PEPCK; PEPCK2; PEPCK-M; K2
Positive Sample:293T,SH-SY5Y,HepG2
Conjugate:Unconjugated
Cellular Localization:Mitochondrion.
Calculated MW:71kDa
Observed MW:71kDa

This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.

Purification Method:Affinity purification
Gene ID:5106
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using [KO Validated] PEPCK/PCK2 Rabbit pAb (CAB21529) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

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