Anti-PELP1 Antibody (CAB3189)

Antibody Name:	Anti-PELP1 Antibody
Antibody SKU:	CAB3189
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1180 of human PELP1 (NP_055204.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, A-549, HeLa, NIH/3T3, Mouse liver, Rat uterus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1180 of human PELP1 (NP_055204.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EVEE GAPP PPTL PPAL PPPE SPPK VQPE PEPE PGLL LEVE EPGT EEER GADT APTL APEA LPSQ GEVE REGE SPAA GPPP QELV EEEP SAPP TLLE EETE DGSD KVQP PPET PAEE EMET ETEA EALQ EKEQ DDTA AMLA DFID CPPD DEKP PPPT EPDS
Gene ID:	27043
Uniprot:	Q8IZL8
Cellular Location:	Cytoplasm, Nucleus, nucleoplasm
Calculated MW:	119kDa
Observed MW:	160kDa

Synonyms:

PELP1, MNAR, P160, proline

Background:

This gene encodes a transcription factor which coactivates transcription of estrogen receptor responsive genes and corepresses genes activated by other hormone receptors or sequence-specific transcription factors. Expression of this gene is regulated by both members of the estrogen receptor family. This gene may be involved in the progression of several types of cancer. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	PELP1: Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence- specific transcription factors. Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner. Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol. Plays a role in E2-mediated cell cycle progression by interacting with RB1. May have important functional implications in ER/growth factor cross-talk. Interacts with several growth factor signaling components including EGFR and HRS. Involved in nuclear receptor signaling via its interaction with AR and NR3C1. May promote tumorigenesis via its interaction with and modulation of several oncogenes including SRC, PI3K, STAT3 and EGFR. Plays a role in cancer cell metastasis via its ability to modulate E2-mediated cytoskeleton changes and cell migration via its interaction with SRC and PI3K. Interacts with HRS, RXRA, SUMO2, HDAC2, RB1 and STAT3. Interacts with PI3K, SRC and EGFR in cytoplasm. Interacts with ESR1 and ESR2 and this interaction is enhanced by 17-beta- estradiol. Interacts with CREBBP, EP300, AR and NR3C1 in a ligand- dependent manner. Forms two complexes in the presence of 17-beta- estradiol; one with SRC and ESR1 and another with LCK and ESR1. Interacts with histone H1 and H3 with a greater affinity for H1. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Isoform 2 is expressed in breast cancer cell lines. Isoform 1 is widely expressed. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 17p13.2 Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nucleus Molecular Function:protein binding; chromatin binding; transcription factor binding Biological Process: transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter
NCBI Summary:	This gene encodes a transcription factor which coactivates transcription of estrogen receptor responsive genes and corepresses genes activated by other hormone receptors or sequence-specific transcription factors. Expression of this gene is regulated by both members of the estrogen receptor family. This gene may be involved in the progression of several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
UniProt Code:	Q8IZL8
NCBI GenInfo Identifier:	115502553
NCBI Gene ID:	27043
NCBI Accession:	Q8IZL8.2
UniProt Secondary Accession:	Q8IZL8,O15450, Q5EGN3, Q6NTE6, Q96FT1, Q9BU60,
UniProt Related Accession:	Q8IZL8
Molecular Weight:	1130
NCBI Full Name:	Proline-, glutamic acid- and leucine-rich protein 1
NCBI Synonym Full Names:	proline, glutamate and leucine rich protein 1
NCBI Official Symbol:	PELP1
NCBI Official Synonym Symbols:	MNAR; P160
NCBI Protein Information:	proline-, glutamic acid- and leucine-rich protein 1; transcription factor HMX3; proline and glutamic acid rich nuclear protein; modulator of non-genomic activity of estrogen receptor
UniProt Protein Name:	Proline-, glutamic acid- and leucine-rich protein 1
UniProt Synonym Protein Names:	Modulator of non-genomic activity of estrogen receptor; Transcription factor HMX3
Protein Family:	Proline-, glutamic acid- and leucine-rich protein
UniProt Gene Name:	PELP1
UniProt Entry Name:	PELP1_HUMAN

	Western blot analysis of extracts of various cell lines, using PELP1 antibody (CAB3189) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of L929 cells using PELP1 Polyclonal Antibody (CAB3189) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using PELP1 Polyclonal Antibody (CAB3189) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-PELP1 Antibody (CAB13414)

Anti-PELP1 Antibody (CAB9026)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)