The PECAM1 Polyclonal Antibody (CAB3181) is a valuable tool for researchers studying PECAM1, a cell adhesion molecule involved in various biological processes, including angiogenesis, platelet activation, and immune response regulation. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications.PECAM1, also known as CD31, is a critical regulator of vascular permeability and leukocyte migration, making it an important target for research in cardiovascular disease, inflammation, and cancer. By specifically binding to the PECAM1 protein, this antibody enables the detection and analysis of PECAM1 expression in different cell types, providing insights into its role in vascular homeostasis and immune cell interactions.
Researchers studying PECAM1-associated pathways and diseases will find this antibody essential for their experiments, as it allows for the precise investigation of PECAM1 expression levels and functions in a wide range of biological contexts. Its high reactivity and specificity make it an ideal tool for immunology, oncology, and vascular biology research.
Product Name:
CD31/PECAM1 Polyclonal Antibody
SKU:
CAB3181
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 630-738 of CD31/PECAM1 (NP_000433.4).
Cell junction, Cell junction, Cell membrane, Lipid-anchor, Single-pass type I membrane protein.
Calculated MW:
81kDa
Observed MW:
130kDa
Predicted to enable protein homodimerization activity; protein phosphatase binding activity; and transmembrane signaling receptor activity. Acts upstream of or within several processes, including Rho protein signal transduction; endothelial cell morphogenesis; and positive regulation of tyrosine phosphorylation of STAT protein. Located in several cellular components, including cell-cell contact zone; external side of plasma membrane; and smooth muscle contractile fiber. Is expressed in several structures, including alimentary system; cardiovascular system; central nervous system; extraembryonic component; and genitourinary system. Human ortholog(s) of this gene implicated in coronary artery disease (multiple); lung non-small cell carcinoma; neuroblastoma; and psoriatic arthritis. Orthologous to human PECAM1 (platelet and endothelial cell adhesion molecule 1).
Purification Method:
Affinity purification
Gene ID:
18613
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from U-937 cells, using CD31/PECAM1 Rabbit pAb (CAB3181) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
