The PEAR1 Polyclonal Antibody (PACO56354) is a vital tool for researchers studying the PEAR1 protein, which is involved in platelet function and cardiovascular health. This antibody, raised in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the PEAR1 protein, this antibody facilitates the detection and analysis of PEAR1 in a variety of cell types, making it an invaluable asset for studies in cardiology and vascular biology.PEAR1, a platelet endothelial aggregation receptor, is essential for platelet activation and aggregation, processes that are crucial for proper blood clot formation and cardiovascular health.
Dysregulation of PEAR1 has been linked to various cardiovascular disorders, making it a promising target for research into heart disease, stroke, and thrombotic conditions. Understanding the role of PEAR1 in platelet function is vital for developing novel therapies aimed at improving cardiovascular outcomes and reducing the risk of thrombotic events.
Western Blot. Positive WB detected in: Rat liver tissue, Mouse kidney tissue. All lanes: PEAR1 antibody at 5.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 111 kDa. Observed band size: 111 kDa.
Immunofluorescence staining of U251 cells with PACO56354 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO56354 diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
When overexpressed, reduces the number of both early and late non-adherent myeloid progenitor cells.
Synonyms:
Platelet endothelial aggregation receptor 1 (hPEAR1) (Multiple epidermal growth factor-like domains protein 12) (Multiple EGF-like domains protein 12), PEAR1, MEGF12
UniProt Protein Function:
PEAR1: When overexpressed, reduces the number of both early and late non-adherent myeloid progenitor cells. Belongs to the MEGF family.Protein type: Receptor, misc.; Membrane protein, integralChromosomal Location of Human Ortholog: 1q23.1
UniProt Protein Details:
NCBI Summary:
PEAR1 is a platelet receptor that signals upon the formation of platelet-platelet contacts independent of platelet activation and secondary to platelet aggregation (Nanda et al., 2005 [PubMed 15851471]).[supplied by OMIM, Mar 2008]
