The Peak1 Polyclonal Antibody (PACO60372) is a reliable tool for research involving the Peak1 protein, a key player in multiple cellular processes such as cell migration and invasion. This antibody, produced in rabbits, has high specificity for human samples and has been validated for use in Western blot applications. It binds specifically to the Peak1 protein, allowing for its detection and analysis in various cell types.Peak1, also known as p130Cas-associated protein, is involved in signaling pathways that regulate cell adhesion, spreading, and motility. Its role in promoting cell migration and invasion makes it a promising target for studies in cancer research and developmental biology.
Understanding the function of Peak1 can provide valuable insights into the mechanisms underlying tumor metastasis and tissue remodeling.Overall, the Peak1 Polyclonal Antibody is a valuable tool for researchers studying cell biology, cancer biology, and other related fields. Its high reactivity and specificity make it ideal for investigating the functions of the Peak1 protein and its implications in physiological and pathological processes.
Antibody Name:
PEAK1 Antibody (PACO60372)
Antibody SKU:
PACO60372
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:
Human
Immunogen:
Recombinant Human Pseudopodium-enriched atypical kinase 1 protein (715-881AA)
IHC image of PACO60372 diluted at 1:200 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN.
PEAK1: a cytoskeleton-associated non-receptor tyrosine kinase that localizes to focal adhesions and actin filaments. Operates downstream of integrin and epidermal growth factor receptors, regulating the P130Cas-Crk-PXN and MAPK signaling. In the late stages of EGF signaling, SGK269 mediates Shc1 protein interactions involved in and cell spreading, migration, and morphogenesis. Amplified in a large fraction of colon cancer. Influences anchorage-independent growth and tumor progression in mice.Protein type: EC 2.7.10.2; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; NKF3 familyChromosomal Location of Human Ortholog: 15q24.3Cellular Component: actin cytoskeleton; cytoplasm; focal adhesion; nucleoplasm; plasma membraneMolecular Function: ATP binding; non-membrane spanning protein tyrosine kinase activity; protein bindingBiological Process: cell migration; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation
UniProt Protein Details:
NCBI Summary:
This gene encodes a non-receptor tyrosine kinase that is a member of the new kinase family three (NFK3) family. In migrating cells, the encoded protein is associated with the actin cytoskeleton and focal adhesions and promotes developing focal adhesion elongation. This protein may play a role in the regulation of cell migration, proliferation and cancer metastasis. [provided by RefSeq, Mar 2014]
