The Peak1 Antibody (PACO22664) is a highly specific and sensitive tool for research involving the Peak1 protein, a key regulator of cell migration and invasion. This polyclonal antibody, produced in rabbits, is optimized for use in a variety of applications, including Western blot, immunofluorescence, and immunohistochemistry.Peak1 is known for its role in promoting cell motility and invasive behavior, making it a crucial target in cancer research and metastasis studies. The Peak1 Antibody binds specifically to the Peak1 protein, allowing for precise detection and analysis in cell lines and tissue samples.
Its high reactivity with human samples ensures accurate results in experiments related to cell migration and invasion mechanisms.Researchers in the fields of oncology, cell biology, and drug discovery will find the Peak1 Antibody to be a valuable tool in their studies. By understanding the function of Peak1 and its impact on cell behavior, new insights into cancer progression and therapeutic approaches can be achieved. This antibody is a reliable resource for investigating the molecular mechanisms underlying metastasis and identifying potential targets for anti-cancer therapies.
Antibody Name:
PEAK1 Antibody (PACO22664)
Antibody SKU:
PACO22664
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from N-terminal of human SGK269.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from 3T3 cells, using SGK269 antibody.
Background:
Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN.
PEAK1: a cytoskeleton-associated non-receptor tyrosine kinase that localizes to focal adhesions and actin filaments. Operates downstream of integrin and epidermal growth factor receptors, regulating the P130Cas-Crk-PXN and MAPK signaling. In the late stages of EGF signaling, SGK269 mediates Shc1 protein interactions involved in and cell spreading, migration, and morphogenesis. Amplified in a large fraction of colon cancer. Influences anchorage-independent growth and tumor progression in mice.Protein type: EC 2.7.10.2; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; NKF3 familyChromosomal Location of Human Ortholog: 15q24.3Cellular Component: actin cytoskeleton; cytoplasm; focal adhesion; nucleoplasm; plasma membraneMolecular Function: ATP binding; non-membrane spanning protein tyrosine kinase activity; protein bindingBiological Process: cell migration; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation
UniProt Protein Details:
NCBI Summary:
This gene encodes a non-receptor tyrosine kinase that is a member of the new kinase family three (NFK3) family. In migrating cells, the encoded protein is associated with the actin cytoskeleton and focal adhesions and promotes developing focal adhesion elongation. This protein may play a role in the regulation of cell migration, proliferation and cancer metastasis. [provided by RefSeq, Mar 2014]
