The PE Rat IgG2a Isotype Control (B39-4) is a crucial tool for researchers conducting flow cytometry experiments involving rat samples. This isotype control is specifically designed to provide a baseline for non-specific binding in flow cytometry assays, ensuring accurate and reliable results. With its PE fluorochrome conjugation, the B39-4 isotype control can be easily distinguished from target antibodies, making it an essential component for immunophenotyping and cell sorting experiments.The B39-4 isotype control offers researchers a standardized reference for assessing background signal in flow cytometry analyses, enabling the precise identification and quantification of target cell populations.
Utilizing this isotype control in conjunction with primary antibodies helps distinguish true antigen-specific binding from non-specific interactions, enhancing the accuracy and specificity of flow cytometry data.Whether studying immune cell subsets, characterizing cell surface markers, or investigating signaling pathways, the PE Rat IgG2a Isotype Control (B39-4) serves as a reliable tool for minimizing false-positive results and ensuring the validity of flow cytometry findings. Researchers can depend on this isotype control to support the rigorous and reproducible analysis of rat-derived samples in various immunological and biomedical research applications.
Product Name:
PE Rat IgG2a, λ Isotype Control [B39-4] (AGEL2893)
Product Code:
AGEL2893
Clone No:
B39-4
Host Species:
Rat
Applications:
FCM
Isotype:
Rat IgG2a, λ
Conjugation:
PE
Form:
Liquid
Fluorescent Label:
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.