The PE Mouse IgG2a Isotype Control (C1.18.4) is a vital tool for researchers conducting flow cytometry experiments. This isotype control antibody is designed specifically for use in mouse samples to help distinguish non-specific background signal from true positive staining in immunofluorescence assays. By binding nonspecifically to cells via Fc receptors, this isotype control allows researchers to accurately interpret and analyze their experimental results without interference from non-specific binding of primary antibodies.Flow cytometry is a powerful technique used in immunology, oncology, and other fields to analyze and quantify the characteristics of individual cells within a heterogeneous population. The PE Mouse IgG2a Isotype Control (C1.18.4) serves as a critical control to ensure the specificity and accuracy of staining in flow cytometry experiments, ultimately leading to reliable and reproducible results.
Researchers can use this isotype control alongside their primary antibodies to confidently identify and characterize cell populations of interest, thereby advancing their understanding of various biological processes and disease mechanisms. With its high quality and specificity, the PE Mouse IgG2a Isotype Control (C1.18.4) from Assay Genie is an essential component for researchers conducting flow cytometry studies involving mouse samples. By providing a reliable means of distinguishing specific signal from background noise, this isotype control facilitates the accurate interpretation of experimental data, ultimately contributing to the advancement of scientific knowledge and the development of innovative therapies for a wide range of diseases and conditions.
Product Name:
PE Mouse IgG2a, κ Isotype Control [C1.18.4] (AGEL2573)
Product Code:
AGEL2573
Clone No:
C1.18.4
Host Species:
Mouse
Applications:
FCM
Isotype:
Mouse IgG2a, κ
Conjugation:
PE
Form:
Liquid
Fluorescent Label:
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.