The PE-Cyanine7 Rat IgG2a Isotype Control (2A3) is a vital tool for flow cytometry experiments requiring an isotype control for rat IgG2a antibodies. This control antibody, manufactured by Assay Genie (AGEL2646), ensures accurate interpretation of experimental results by providing a baseline for non-specific binding in samples. With its PE-Cyanine7 conjugation, this isotype control is ideal for use in multi-color flow cytometry panels, enabling researchers to differentiate specific staining from background noise.Flow cytometry is a powerful technique used in immunology, oncology, and various research fields to analyze and characterize cells based on their surface markers.
By using the PE-Cyanine7 Rat IgG2a Isotype Control (2A3), researchers can confidently distinguish true positive signals and assess the specificity of their antibody staining. This control antibody is essential for maintaining the integrity and reliability of flow cytometry data, ultimately leading to more accurate and reproducible experimental outcomes.
Product Name:
PE/Cyanine7 Rat IgG2a, κ Isotype Control [2A3] (AGEL2646)
Product Code:
AGEL2646
Clone No:
2A3
Host Species:
Rat
Applications:
FCM
Isotype:
Rat IgG2a, κ
Conjugation:
PE/Cyanine7
Form:
Liquid
Fluorescent Label:
PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter).
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.