The PE-Cyanine7 Rat IgG1 Isotype Control (HRPN-AGEL2622) available from Assay Genie is a critical tool for ensuring the specificity and accuracy of your flow cytometry experiments. This isotype control antibody, specifically designed for use in rat samples, serves as a crucial negative control in flow cytometry panels by mimicking the non-specific binding and background signals that can occur during staining procedures.Flow cytometry is a powerful technique used in immunology, cell biology, and cancer research to analyze and quantify properties of individual cells within a heterogeneous population. By including the PE-Cyanine7 Rat IgG1 Isotype Control in your flow cytometry experiments, you can differentiate between specific antibody staining and non-specific interactions, ensuring that any signal detected is a result of true antigen-antibody binding.
This isotype control antibody is conjugated to PE-Cyanine7, a bright and stable fluorophore suitable for multicolor flow cytometry applications. By using this control alongside your experimental antibody panels, you can confidently identify and gate cells of interest, distinguish signal from noise, and draw accurate conclusions from your flow cytometry data.Trust Assay Genie's PE-Cyanine7 Rat IgG1 Isotype Control to provide you with reliable and reproducible results in your flow cytometry studies. Whether you are investigating immune cell populations, studying cell signaling pathways, or characterizing tumor cells, this control antibody ensures the integrity of your flow cytometry data, ultimately leading to more robust and meaningful research outcomes.
Product Name:
PE/Cyanine7 Rat IgG1, κ Isotype Control [HRPN] (AGEL2622)
Product Code:
AGEL2622
Clone No:
HRPN
Host Species:
Rat
Applications:
FCM
Isotype:
Rat IgG1, κ
Conjugation:
PE/Cyanine7
Form:
Liquid
Fluorescent Label:
PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter).
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.