The PE-Cyanine5 Rat IgM Isotype Control (RTK2118-AGEL2510) offered by Assay Genie serves as a critical tool for flow cytometry experiments, particularly when working with rat samples. This isotype control antibody is specifically designed to match the IgM isotype, providing a reliable baseline for assessing nonspecific binding in flow cytometry assays. With its PE-Cyanine5 fluorescent tag, this control is easily distinguishable and enables researchers to accurately assess the specificity of their primary antibody staining.Flow cytometry is a powerful technique for studying cellular markers and identifying different cell populations within complex samples. By using a suitable isotype control like the PE-Cyanine5 Rat IgM Isotype Control, researchers can distinguish between true positive staining and background noise, ensuring the validity and reliability of their flow cytometry data.
This control antibody is essential for optimizing experimental conditions and obtaining accurate results in flow cytometry-based studies involving rat samples.In summary, the PE-Cyanine5 Rat IgM Isotype Control (RTK2118-AGEL2510) from Assay Genie is a valuable reagent for flow cytometry experiments with rat samples, providing researchers with a reliable tool to assess nonspecific binding and ensure the specificity of their primary antibody staining.
Product Name:
PE/Cyanine5 Rat IgM, κ Isotype Control [RTK2118] (AGEL2510)
Product Code:
AGEL2510
Clone No:
RTK2118
Host Species:
Rat
Applications:
FCM
Isotype:
Rat IgM, κ
Conjugation:
PE/Cyanine5
Form:
Liquid
Fluorescent Label:
PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.