PE/Cyanine5 Mouse IgG3, κ Isotype Control [A112-3]
The PE-Cyanine5 Mouse IgG3 Isotype Control (AGEL2874) is a vital tool for flow cytometry experiments requiring precise control and validation of antibody binding specificity. This isotype control antibody, derived from mouse IgG3, is utilized as a reference point to differentiate non-specific background signals from specific antibody staining in immunophenotyping experiments. It allows researchers to establish baseline fluorescence parameters and set gating strategies to accurately identify positive cell populations in their samples.This PE-Cyanine5 Mouse IgG3 Isotype Control (AGEL2874) serves as an essential component in flow cytometry panels by providing a reliable negative control to assess the specificity of antibody binding and ensure the accuracy of experimental results.
By incorporating this isotype control in flow cytometry studies, researchers can confidently interpret their data, validate their staining protocols, and enhance the reproducibility of their findings. This product is particularly valuable for immunologists, cell biologists, and researchers working on immune cell characterization, biomarker discovery, and immune response profiling.
Product Name:
PE/Cyanine5 Mouse IgG3, κ Isotype Control [A112-3] (AGEL2874)
Product Code:
AGEL2874
Clone No:
A112-3
Host Species:
Mouse
Applications:
FCM
Isotype:
Mouse IgG3, κ
Conjugation:
PE/Cyanine5
Form:
Liquid
Fluorescent Label:
PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.