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PE Anti-Mouse CD34 Antibody [RAM34] (AGEL3005)

SKU:
AGEL3005
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Applications:
FCM
Reactivity:
Mouse
Host Species:
Rat
Isotype:
Rat IgG2a, κ
Clone:
RAM34
Conjugation:
PE
€179 - €459
Frequently bought together:

Description

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PE Anti-Mouse CD34 Antibody [RAM34]

The PE Anti-Mouse CD34 Antibody (clone RAM34) is a vital tool for researchers studying hematopoietic stem cells and vascular endothelial cells in mouse models. This antibody, produced by Assay Genie, specifically targets CD34, a transmembrane glycoprotein involved in cell adhesion and migration.CD34 is widely used as a marker for identifying hematopoietic stem cells and progenitor cells in various tissues. The PE Anti-Mouse CD34 Antibody (RAM34) is validated for flow cytometry applications, allowing for precise identification and characterization of CD34-positive cell populations. Its high specificity and sensitivity make it an essential reagent for studies on stem cell biology, tissue regeneration, and vascular development in mouse models.

By utilizing the PE Anti-Mouse CD34 Antibody (RAM34), researchers can enhance their understanding of hematopoiesis, angiogenesis, and tissue regeneration processes. This antibody enables accurate and reliable detection of CD34 expression in various cell types, providing valuable insights into the role of CD34 in different physiological and pathological conditions. Trusted for its quality and performance, this antibody from Assay Genie facilitates cutting-edge research in the fields of stem cell biology, regenerative medicine, and vascular biology.

Product Title:PE Anti-Mouse CD34 Antibody [RAM34]
SKU:AGEL3005
Size:25µg, 100µg
Clone No:RAM34
Synonyms:Hematopoietic progenitor cell antigen CD34;
Host Species:Rat
Reactivity:Mouse
Application:FCM
Isotype:Rat IgG2a, κ
Isotype Control:PE Rat IgG2a, κ Isotype Control[2A3]
Form:Liquid
Conjugation:PE
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:Spectrum Image
Swissprot:Q64314
Gene ID:12490
Storage Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:The RAM34 antibody reacts with the CD34 glycoprotein on the surface of three independently derived mouse CD34-transfected cell lines. RAM34 antibody also reacts with the mouse cell lines PA6, 416B, Swiss 3T6, NIH, 3T3, DA1, and M1, all of which are positive for expression of mouse CD34 mRNA. Cell lines shown to be negative for CD34 transcript, including WEHI-3B, EL4, 18.8, and CMT64/61, are also negative for surface expression of CD34 as determined by RAM34 staining. Normal thymocytes and splenocytes are negative for CD34 expression. In the bone marrow, 7-10% of cells are stained with RAM34 mAb, including most of the Ly-6A/E (Sca-1)+ CD90 (Thy-1)low Lineage Marker- hematopoietic stem cell-enriched subpopulation and myeloerythroid progenitors. CD34 is also expressed on a small percentage of fetal liver cells, including NK-cell progenitors. CD34 has been reported to be expressed on the endothelium of capillaries and, in this form, to function as a ligand for L-selectin. Consistent with this observation, RAM34 antibody stains endothelial cells in spleen, thymus, and postcapillary HEVs in the lymph nodes. It is reported that RAM34 antibody can be used to select CD34+ CD117 (c-Kit)+ Ly-6A/E (Sca-1)+ Lineage Marker- bone marrow-derived hematopoietic stem cells, capable of short-term multi-lineage reconstitution of lethally irradiated mice; while the CD34- CD117+ Sca-1+ Lineage Marker- population contains self-renewing hematopoietic stem cells. Similarly, the bone marrow population with high dye-efflux capacity and which is highly enriched for long-term reconstituting hematopoietic stem cells is CD34- CD117 (c-Kit)+ Ly-6A/E (Sca-1)+ Lineage Marker-.

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