The PDX1 Polyclonal Antibody (CAB10173) is a valuable tool for research involving the PDX1 protein, a transcription factor essential for pancreatic development and beta cell function. This antibody, produced in rabbits, exhibits strong reactivity with human samples and has been validated for use in various applications, including Western blot and immunohistochemistry.PDX1, also known as insulin promoter factor 1, plays a crucial role in regulating the expression of genes involved in insulin production and glucose metabolism.
Dysregulation of PDX1 has been implicated in the pathogenesis of diabetes and pancreatic cancer, making it a key target for studies in endocrinology and oncology research. By detecting and analyzing PDX1 protein expression in different cell types and tissues, researchers can gain insights into the molecular mechanisms underlying pancreatic function and disease. This antibody is an essential tool for studying the role of PDX1 in pancreatic biology and for developing potential therapies targeting this transcription factor.
Product Name:
PDX1 Rabbit Polyclonal Antibody
SKU:
CAB10173
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PDX1 (NP_000200.1).
The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (IDDM), as well as maturity onset diabetes of the young type 4 (MODY4).
Purification Method:
Affinity purification
Gene ID:
3651
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PDX1 antibody (CAB10173) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
