The PDS5B Polyclonal Antibody (PAC015822) is a valuable tool for researchers studying the PDS5B protein, which plays a key role in DNA repair and chromosome segregation processes. This antibody, raised in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By targeting the PDS5B protein, researchers can investigate its function and localization in various cell types, making it ideal for studies in genetics and cancer research.PDS5B is essential for maintaining genomic stability and ensuring accurate chromosome segregation during cell division. Dysregulation of PDS5B has been linked to various genetic disorders and cancers, highlighting its importance in cell cycle regulation.
By understanding the role of PDS5B, researchers can gain insights into the mechanisms underlying cancer development and potentially identify new therapeutic targets for treatment.Overall, the PDS5B Polyclonal Antibody is a reliable tool for investigating the function of PDS5B in biological processes, offering valuable insights into the molecular mechanisms governing DNA repair and chromosome dynamics. Its specificity and sensitivity make it a valuable asset for researchers seeking to unravel the complexities of genomic stability and its implications for human health.
Antibody Name:
PDS5B Antibody (PACO15822)
Antibody SKU:
PACO15822
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:1000-1:2000, WB:1:200-1:1000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Fusion protein of human PDS5B
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: Hela cells, Primary antibody: PACO15822(PDS5B Antibody) at dilution 1/316.6, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds.
Background:
This gene encodes a protein that interacts with the conserved protein complex termed cohesion. The cohesion complex holds together sister chromatids and facilitates accurate chromosome segregation during mitosis and meiosis. This protein is also a negative regulator of cell proliferation and may be a tumor-suppressor gene.
Synonyms:
PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae)
UniProt Protein Function:
APRIN: a widely expressed nuclear protein. Is lost in many cancers and may function as a tumor suppressor. Correlates with differentiation in embryonal carcinoma stem cells; its knockdown disrupted Nanog, Oct4, and SOX2 regulation. Plays a role in androgen-induced proliferative arrest in prostate cells. Is upregulated in androgen-sensitive LNCaP prostate cancer cells undergoing androgen-induced proliferative block. Required for maintenance of sister chromatid cohesion during mitosis. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Belongs to the PDS5 family. Induced by the synthetic androgen R1881 in prostate carcinoma cells undergoing proliferative arrest. Phosphorylated upon DNA damage. Maximum levels occur 18-20 hours after androgen exposure. Interacts with the cohesin complex. Phosphorylated upon DNA damage, probably by ATM or ATR. Five isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Cell development/differentiation; DNA-binding
Chromosomal Location of Human Ortholog: 13q12.3
Cellular Component: nucleoplasm; chromosome; nucleus; chromatin; cytosol; chromosome, pericentric region
Molecular Function:protein binding; DNA binding; ATP binding
This gene encodes a protein that interacts with the conserved protein complex termed cohesin. The cohesin complex holds together sister chromatids and facilitates accurate chromosome segregation during mitosis and meiosis. This protein is also a negative regulator of cell proliferation and may be a tumor-suppressor gene. [provided by RefSeq, Jul 2015]
