The PDP1 Polyclonal Antibody (CAB14545) is a valuable tool for researchers studying the protein PDP1, which plays a role in metabolism and mitochondrial function. This antibody, produced in rabbits, is highly specific for human samples and is validated for use in applications such as Western blotting. By binding to the PDP1 protein, this antibody allows for the detection and analysis of PDP1 in various cell types, making it ideal for studies in the fields of biochemistry and cellular metabolism.PDP1, also known as pyruvate dehyrogenase phosphatase subunit 1, is a crucial enzyme involved in the regulation of glucose metabolism and energy production within cells.
Dysregulation of PDP1 activity has been linked to various metabolic disorders, making it a promising target for research into conditions such as diabetes, obesity, and cardiovascular disease. By understanding the role of PDP1, researchers can gain insights into the mechanisms underlying these diseases and potentially develop new therapeutic strategies.
Product Name:
PDP1 Rabbit Polyclonal Antibody
SKU:
CAB14545
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 72-260 of human PDP1 (NP_060914.2).
Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
54704
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PDP1 Rabbit pAb (CAB14545) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
