The PDK4 Polyclonal Antibody (CAB3069) is a valuable tool for researchers studying the pyruvate dehydrogenase kinase 4 (PDK4) protein, which plays a key role in regulating the metabolism of glucose and fatty acids in cells. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications.PDK4 is known to be involved in metabolic processes that control energy production and storage, making it a target of interest in studies of metabolic diseases such as diabetes and obesity. By binding specifically to the PDK4 protein, this antibody allows for the detection and analysis of PDK4 levels in various cell types, providing important insights into how this enzyme modulates cellular metabolism.
Research using the PDK4 Polyclonal Antibody can contribute to a better understanding of the role of PDK4 in metabolic regulation, potentially leading to the development of new therapeutic strategies for metabolic disorders. The antibody is a valuable tool for investigations in the fields of metabolism, diabetes, and obesity research.
Product Name:
PDK4 Rabbit Polyclonal Antibody
SKU:
CAB3069
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 312-411 of human PDK4 (NP_002603.1).
This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.
Purification Method:
Affinity purification
Gene ID:
5166
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PDK4 Rabbit pAb (CAB3069) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
