The PDK3 Polyclonal Antibody (CAB8028) is a valuable tool for researchers studying the protein pyruvate dehydrogenase kinase 3 (PDK3). This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications. By binding to the PDK3 protein, this antibody enables the detection and analysis of PDK3 levels in various cell types, making it ideal for studies in metabolic regulation and cancer research.PDK3 is a key enzyme involved in the regulation of glucose metabolism, specifically in the inhibition of pyruvate dehydrogenase complex. Dysregulation of PDK3 has been implicated in various metabolic disorders and cancer, making it a potential target for therapeutic interventions.
By investigating the role of PDK3 in cellular metabolism, researchers can gain insights into disease mechanisms and potentially identify new treatment strategies.Overall, the PDK3 Polyclonal Antibody is a valuable tool for researchers interested in exploring the function of PDK3 in health and disease. Its high specificity and sensitivity make it a reliable choice for studying the role of this critical enzyme in metabolic pathways and potential therapeutic interventions.
Product Name:
PDK3 Rabbit Polyclonal Antibody
SKU:
CAB8028
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 137-406 of human PDK3 (NP_005382.1).
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5165
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PDK3 antibody (CAB8028) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
