The PDK2 Polyclonal Antibody (CAB4012) is a specialized tool for researchers studying pyruvate dehydrogenase kinase 2 (PDK2), a key enzyme involved in regulating the production of ATP in cells. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.PDK2 plays a crucial role in the regulation of cellular metabolism, particularly in the context of cancer and metabolic disorders. By targeting PDK2, researchers can gain insights into the mechanisms that drive abnormal cell growth and energy metabolism in these diseases.
The PDK2 Polyclonal Antibody offers a reliable means of detecting and analyzing PDK2 protein levels in various cell types, making it an essential tool for studies in cancer biology and metabolic research.Understanding the function of PDK2 is key to uncovering new therapeutic targets and treatment strategies for diseases characterized by dysregulated metabolism. By using the PDK2 Polyclonal Antibody, researchers can delve deeper into the role of this enzyme in health and disease, paving the way for innovative therapies that target metabolic pathways in cancer and other metabolic disorders.
Product Name:
PDK2 Rabbit Polyclonal Antibody
SKU:
CAB4012
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 128-407 of human PDK2 (NP_002602.2).
This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
5164
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PDK2 antibody (CAB4012) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
