The PDK1 Polyclonal Antibody (CAB0834) is a valuable tool for researchers studying PDK1, a key regulator of cell growth and metabolism. This antibody is raised in rabbits and specifically detects the PDK1 protein in human samples, making it ideal for Western blot applications.PDK1, also known as pyruvate dehydrogenase kinase 1, is an enzyme that plays a crucial role in the regulation of glucose metabolism and cell survival. Dysregulation of PDK1 has been implicated in various diseases, including cancer, diabetes, and cardiovascular disorders.
By using the PDK1 Polyclonal Antibody, researchers can analyze the expression and activity of PDK1 in different cell types, providing valuable insights into its function and potential therapeutic targets.With its high specificity and sensitivity, the PDK1 Polyclonal Antibody is a reliable tool for studies in cell biology, metabolism, and disease mechanisms. By uncovering the role of PDK1 in various cellular processes, researchers can pave the way for the development of novel treatments and interventions for a range of diseases.
Product Name:
PDK1 Rabbit Polyclonal Antibody
SKU:
CAB0834
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 337-436 of human PDK1/PDHK1 (NP_002601.1).
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5163
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PDK1/PDHK1 antibody (CAB0834) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
