The PDHB Rabbit Monoclonal Antibody (CAB4645) is a valuable tool for researchers studying the Pyruvate Dehydrogenase E1 Component Subunit Beta (PDHB) protein. This monoclonal antibody, developed using rabbit-derived cells, is highly specific and reactive to human PDHB samples, making it ideal for a variety of research applications.PDHB is a critical component of the pyruvate dehydrogenase complex, which plays a key role in cellular metabolism and energy production. Dysregulation of PDHB has been linked to various diseases, including cancer, diabetes, and neurodegenerative disorders. The PDHB Rabbit Monoclonal Antibody enables precise detection and analysis of PDHB in different cell types, providing researchers with a reliable tool for exploring its function and potential therapeutic targets.
By utilizing the PDHB Rabbit Monoclonal Antibody in experiments such as Western blotting, researchers can gain insights into the role of PDHB in cellular metabolism and its implications in disease pathology. This antibody is a valuable asset for studies in biochemistry, molecular biology, and metabolic disorders, offering a deeper understanding of PDHB and its clinical significance.
Product Name:
PDHB Rabbit Monoclonal Antibody
SKU:
CAB4645
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 260-359 of human Pyruvate Dehydrogenase E1 beta subunit (P11177).
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and carbon dioxide, and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 beta subunit. Mutations in this gene are associated with pyruvate dehydrogenase E1-beta deficiency. Alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5162
Clone Number:
ARC1074
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Pyruvate Dehydrogenase E1 beta subunit Rabbit mAb (CAB4645) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
