The PDHA1 Rabbit Monoclonal Antibody is a cutting-edge research tool designed for the study of Pyruvate Dehydrogenase E1 Alpha 1 (PDHA1), an essential enzyme involved in cellular energy production. This antibody, developed using rabbit monoclonal technology, exhibits high specificity and sensitivity towards PDHA1 protein in human samples, making it an invaluable tool for various research applications.PDHA1 plays a crucial role in the conversion of pyruvate to acetyl-CoA, a key step in the citric acid cycle and aerobic respiration. Dysregulation of PDHA1 function has been implicated in various metabolic disorders and neurodegenerative diseases, highlighting the significance of studying this enzyme in both basic and clinical research settings.
By utilizing the PDHA1 Rabbit Monoclonal Antibody, researchers can accurately detect and analyze PDHA1 in different cell types and tissues, opening up new possibilities for understanding its role in metabolism, disease development, and potential therapeutic interventions. Whether investigating mitochondrial function, metabolic pathways, or disease mechanisms, this antibody is a valuable tool for advancing scientific knowledge and biomedical research.
Product Name:
PDHA1 Rabbit Monoclonal Antibody
SKU:
CAB13687
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PDHA1 (P08559).
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5160
Clone Number:
ARC0722
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using PDHA1 Rabbit mAb (CAB13687) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
