PDHA1 Rabbit Polyclonal Antibody (CAB1895)
- SKU:
- CAB1895
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
Product Name: | PDHA1 Rabbit Polyclonal Antibody |
SKU: | CAB1895 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-390 of human PDHA1 (NP_000275.1). |
Sequence: | FAND ATFE IKKC DLHR LEEG PPVT TVLT REDG LKYY RMMQ TVRR MELK ADQL YKQK IIRG FCHL CDGQ EACC VGLE AGIN PTDH LITA YRAH GFTF TRGL SVRE ILAE LTGR KGGC AKGK GGSM HMYA KNFY GGNG IVGA QVPL GAGI ALAC KYNG KDEV CLTL YGDG AANQ GQIF EAYN MAAL WKLP CIFI CENN RYGM GTSV ERAA ASTD YYKR GDFI PGLR VDGM DILC VREA TRFA AAYC RSGK GPIL MELQ TYRY HGHS MSDP GVSY RTRE EIQE VRSK SDPI MLLK DRMV NSNL ASVE ELKE IDVE VRKE IEDA AQFA TADP EPPL EELG YHIY SSDP PFEV RGAN QWIK FKSV S |
Tested Applications: | WB IHC-P IF/ICC IP ELISA |
Recommended Dilution: | WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
Synonyms: | PDHA; PDHAD; PHE1A; E1alpha; PDHCE1A; PDHA1 |
Positive Sample: | HepG2,293T,HeLa,NIH/3T3,Neuro-2a,PC-12 |
Conjugate: | Unconjugated |
Cellular Localization: | Mitochondrion matrix. |
Calculated MW: | 43kDa |
Observed MW: | 43kDa |
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method: | Affinity purification |
Gene ID: | 5160 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |