The PDHA1 Polyclonal Antibody (CAB1895) is a valuable tool for researchers studying the pyruvate dehydrogenase E1 alpha subunit (PDHA1) protein. This antibody, produced in rabbits, is highly specific to human samples and is validated for Western blot applications. It binds specifically to the PDHA1 protein, allowing for accurate detection and analysis in various cell types.PDHA1 is a crucial enzyme involved in the conversion of pyruvate to acetyl-CoA, a key step in the process of cellular energy production. Dysregulation of PDHA1 activity has been linked to various metabolic disorders and diseases, making it an important target for research in areas such as metabolism, diabetes, and neurodegenerative disorders.
By utilizing the PDHA1 Polyclonal Antibody, researchers can gain insights into the function and regulation of PDHA1, ultimately advancing our understanding of cellular metabolism and its implications for human health. This antibody is essential for studies seeking to unravel the complex mechanisms underlying metabolic diseases and develop novel therapeutic strategies.
Product Name:
PDHA1 Rabbit Polyclonal Antibody
SKU:
CAB1895
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-390 of human PDHA1 (NP_000275.1).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
PDHA; PDHAD; PHE1A; E1alpha; PDHCE1A; PDHA1
Positive Sample:
HepG2,293T,HeLa,NIH/3T3,Neuro-2a,PC-12
Conjugate:
Unconjugated
Cellular Localization:
Mitochondrion matrix.
Calculated MW:
43kDa
Observed MW:
43kDa
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5160
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using PDHA1 Rabbit pAb (CAB1895) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 0.5s.
