PDHA1 Monoclonal Antibody (CAB22084)
- SKU:
- CAB22084
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
Description
Product Name: | PDHA1 Monoclonal Antibody |
SKU: | CAB22084 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 248-348 of human PDHA1 (NP_000275.1). |
Sequence: | IPGL RVDG MDIL CVRE ATRF AAAY CRSG KGPI LMEL QTYR YHGH SMSD PGVS YRTR EEIQ EVRS KSDP IMLL KDRM VNSN LASV EELK EIDV EVRK EIED |
Tested Applications: | WB ELISA |
Recommended Dilution: | WB,1:1000 - 1:5000 |
Synonyms: | PDHA; PDHAD; PHE1A; E1alpha; PDHCE1A; PDHA1 |
Positive Sample: | HepG2, NIH/3T3 |
Conjugate: | Unconjugated |
Cellular Localization: | Mitochondrion matrix. |
Calculated MW: | 43kDa |
Observed MW: | 43kDa |
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method: | Affinity purification |
Gene ID: | 5160 |
Clone Number: | ARC53497 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |