PDF Rabbit Polyclonal Antibody (CAB10458)
- SKU:
- CAB10458
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
Product Name: | PDF Rabbit Polyclonal Antibody |
SKU: | CAB10458 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-243 of human PDF (NP_071736.1). |
Sequence: | EGPA LRRS YWRH LRRL VLGP PEPP FSHV CQVG DPVL RGVA APVE RAQL GGPE LQRL TQRL VQVM RRRR CVGL SAPQ LGVP RQVL ALEL PEAL CREC PPRQ RALR QMEP FPLR VFVN PSLR VLDS RLVT FPEG CESV AGFL ACVP RFQA VQIS GLDP NGEQ VVWQ ASGW AARI IQHE MDHL QGCL FIDK MDSR TFTN VYWM KVND |
Tested Applications: | WB ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 |
Synonyms: |
Positive Sample: | MCF7,SW620,A-549,HepG2,K-562,Mouse heart,Rat heart |
Conjugate: | Unconjugated |
Cellular Localization: | Mitochondrion. |
Calculated MW: | 27kDa |
Observed MW: | 20kDa |
Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells.
Purification Method: | Affinity purification |
Gene ID: | 64146 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |