The PDF Polyclonal Antibody (CAB10458) is specifically designed for researchers studying CD300A, a cell surface molecule known for its role in immune regulation. This antibody, generated in rabbits, is highly reactive with human samples and is validated for Western blot applications. By binding to the CD300A protein, this antibody allows for detection and analysis in various cell types, making it an ideal tool for studies in immunology and cancer research.CD300A, also referred to as an immune inhibitory receptor, plays a key role in maintaining immune homeostasis by regulating inflammation and inhibiting allergic reactions.
Its importance in modulating immune responses makes it a valuable target for research into diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions. Understanding the function of CD300A is essential for developing therapies that can either harness or manipulate immune function in these disease contexts.
Product Name:
PDF Rabbit Polyclonal Antibody
SKU:
CAB10458
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 40-243 of human PDF (NP_071736.1).
Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells.
Purification Method:
Affinity purification
Gene ID:
64146
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PDF Rabbit pAb (CAB10458) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
